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Repeat Steps 1B(i)–(ii) to layer the 10 and 3% Percoll layers. Points from here (point 2) up to and including point 5 are related to Preparation of S1 fraction from brain tissueTiming: 30 min Extract the required brain tissue or brain region …
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Worse than John Bolton? Absolutely. Philip Giraldi, a former CIA officer, is executive director of the Council for the National Interest. [This article originally appeared at The Unz Review, reprinted with permission.]
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